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Traces were restricted to TCP traf c only; all user datagram protocol (UDP) traf c was discarded. Thus roughly one-third of all traf c at CMU and on the internal AT&T network was discarded but almost none on the external AT&T network. Note that neither of these networks carried any substantial amount of MBONE traf c. To be able to collect data over a reasonable time period only those TCP packets that are involved in the TCP connection establishment handshakes between the source and destination pair were collected, that is, those packets that either have their synchronize sequence numbers ag (SYN) or their nish ag (FIN) set in the TCP header (ignoring RST packets). From the traces one can derive the arrival time of TCP connections, their source and destination, their application, their durations, and the number of bytes transferred (excluding TCP=IP overhead) using the tcp-conn tool [22]. The CMU datasets cover nearly 161 hours over 8 different days and the AT&T datasets more than 1290 hours over 52 days. The packets with SYN, FIN ags are classi ed according to the application that generated them. We distinguished the traf c classes shown in Table 15.1. This classi cation to applications is based on the port numbers of the packets. All packets with port numbers that t none of these applications are collected in a separate class. Table 15.2 presents a breakdown of the observed TCP connection arrivals according to application classes. First we note that the traf c volume and traf c mix are highly dependent on the kind of network we were monitoring and when we were monitoring them. The observed change in the CMU traf c mix between December 1994 and June 1995 re ects the increasing popularity of the World Wide

1 and Figures 163 166 This nding is in stark contrast to the current univalent (eg, Zicam and strain-speci c vaccination) approaches to preventing viral entry and disease While mPEG grafting will not replace vaccinations against known pathogens, it may prove to be a highly effective approach in the prevention of ill-de ned respiratory viruses during u season or, potentially, viruses unleashed to promote public panic The application of this immunoprotective barrier is surprisingly simple, requiring only a 3 5 minute application of the activated mPEG compound as either a nasal gel or throat spray Our studies demonstrate that near maximal protection by a single application lasts a minimum of 24 hours and still has signi cant protective bene ts up to 48 or even 60 hours postapplication.

TABLE 15.1 X:

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Cells of the immune system include several types of leukocytes (white blood cells) (Table 19.1), which are derived from bone marrow. T lymphocytes, a subset of immune

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Indeed, the immunocamou age of host cells or multiple viral families represents perhaps the only broad-spectrum antiviral approach described to date..

Disclaimer: This chapter has been reviewed by the National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency and approved for publication. Approval does not signify that the contents necessarily re ects the views and policies of the Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. A Textbook of Modern Toxicology, Third Edition, edited by Ernest Hodgson ISBN 0-471-26508-X Copyright 2004 John Wiley & Sons, Inc.

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Figure 19.1 Potential consequences of immunotoxicity. Table 19.1 Leukocytes Granulocytes (polymorphonuclear leukocytes) Neutrophils Eosinophils Basophils/mast cellsa Monocytes Lymphocytes Monocytes/macrophagesa Natural killer cells

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ACKNOWLEDGMENTS This work was completed in partial ful llment of a doctoral thesis (by L.L.M.) to ful ll the requirements of the Graduate Program in Pathology and Laboratory Medicine at the University of British Columbia. This work was supported by the Canadian Blood Services and the Canadian Institutes of Health Research.

cells, undergo differentiation and maturation in the thymus. Leukocytes circulate throughout the body in blood and lymph and populate other lymphoid tissues including the spleen, lymph nodes (scattered throughout the body), tonsils, and adenoids, as well as aggregates of lymphoid tissue in the lung, gut, and skin, which are referred to as bronchus-, gut- and skin-associated lymphoid tissue (BALT, GALT, and SALT). Also immune cells can be recruited to almost any tissue in the body where there is injury or infection. Accumulation of leukocytes in tissues in response to injury is known as in ammation. Cytokines (e.g., interleukins, interferons, and chemokines), soluble mediators produced by immune cells as well as cells outside the immune system, control the maturation, differentiation, and mobilization of immune cells. Immune responses are divided into innate responses directed nonspeci cally against foreign substances, and acquired responses directed against speci c antigens. There is considerable interaction between these two types of immunity. Innate immunity provides a rapid, although usually incomplete, antimicrobial defense. Granulocytes, natural killer cells, and macrophages are important mediators of innate immunity. Granulocytes have the capacity to phagocytize (engulf) infectious agents or other types of particles and to destroy or remove them from the tissue. They release a variety of soluble mediators that can kill invading organisms, increase vascular permeability, and recruit more leukocytes to the tissue. Natural killer cells are large granular lymphocytes that nonspeci cally kill tumor and virus-infected cells. Macrophages are also phagocytic, can release chemotactic and cytotoxic cytokines, and, when activated, can kill tumor or virus-infected cells. Mediators released from

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